期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 11, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2021.696432
关键词
peri-implantitis; saliva; microbiota (16S); host-bacteria interaction; dysbioses
资金
- Sao Paulo Research Foundation (FAPESP) [2013/08242-3, 2015/07727-9]
- Brazilian Coordination for the Improvement of Higher Education Personnel (CAPES) [001]
- National Council for Scientific and Technological Development (CNPq) [311368/2019-0, 44004/2014-5]
- National Institute of Health Research (NIHR CL)
- National Funding for Scientific and Technologic Development of Chile (FONDECYT) [3210687]
- Universidad de La Frontera (DIUFRO) [DIM20-0019]
- Universidad de La Frontera [VRIP20P002]
Differences in salivary microbiome composition were observed between patients with healthy peri-implant sites and those with peri-implantitis. Bleeding on probing may affect the diversity of the salivary microbiome.
Background and ObjectivesThe aim of this study was to examine the salivary microbiome in healthy peri-implant sites and those with peri-implantitis. MethodsSaliva samples were collected from 21 participants with healthy peri-implant sites and 21 participants with peri-implantitis. The V4 hypervariable region of the 16S rRNA gene was sequenced using the Ion Torrent PGM System (Ion 318 (TM) Chip v2 400). The NGS analysis and composition of the salivary microbiome were determined by taxonomy assignment. Downstream bioinformatic analyses were performed in QIIME (v 1.9.1). ResultsClinical differences according to peri-implant condition status were found. Alpha diversity metrics revealed that the bacterial communities of participants with healthy peri-implant sites tended to have a richer microbial composition than individuals with peri-implantitis. In terms of beta diversity, bleeding on probing (BoP) may influence the microbial diversity. However, no clear partitioning was noted between the salivary microbiome of volunteers with healthy peri-implant sites or volunteers with peri-implantitis. The highest relative abundance of Stenotrophomonas, Enterococcus and Leuconostoc genus, and Faecalibacterium prausnitzii, Haemophilus parainfluenzae, Prevotella copri, Bacteroides vulgatus, and Bacteroides stercoris bacterial species was found in participants with peri-implantitis when compared with those with healthy peri-implant sites. ConclusionDifferences in salivary microbiome composition were observed between patients with healthy peri-implant sites and those with peri-implantitis. BoP could affect the diversity (beta diversity) of the salivary microbiome.
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