Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons

Somatosensory neurons with cell bodies in the dorsal root ganglia (DRG) project to the skin, muscles, bones, and viscera to detect touch and temperature as well as to mediate proprioception and many types of interoception. In addition, the somatosensory system conveys the clinically relevant noxious sensations of pain and itch. Here, we used single nuclear transcriptomics to characterize transcriptomic classes of human DRG neurons that detect these diverse types of stimuli. Notably, multiple types of human DRG neurons have transcriptomic features that resemble their mouse counterparts although expression of genes considered important for sensory function often differed between species. More unexpectedly, we identified several transcriptomic classes with no clear equivalent in the other species. This dataset should serve as a valuable resource for the community, for example as means of focusing translational efforts on molecules with conserved expression across species.

Automated summary

Through single nuclear transcriptomics of human DRG neurons, it was found that different types of neurons share similarities in their transcriptomic features, but there are species differences in the expression of genes related to sensory function. Surprisingly, several transcriptomic classes without clear equivalents in other species were identified.