期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.68135
关键词
epithelial organisation; Euler's formula; Lewis' law; Aboav-Weaire's law; physical principles; lightsheet microscopy; Mouse
类别
资金
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung [CRSII5_170930]
- Swiss National Science Foundation (SNF) [CRSII5_170930] Funding Source: Swiss National Science Foundation (SNF)
Research has shown that during growth, pseudostratified epithelial cells exhibit highly irregular 3D shapes and dynamic organisation through neighbour intercalations. The cell packing configurations across the tissue thickness follow fundamental relationships described previously, minimizing lateral cell-cell surface energy.
During morphogenesis, epithelial sheets remodel into complex geometries. How cells dynamically organise their contact with neighbouring cells in these tightly packed tissues is poorly understood. We have used light-sheet microscopy of growing mouse embryonic lung explants, three-dimensional cell segmentation, and physical theory to unravel the principles behind 3D cell organisation in growing pseudostratified epithelia. We find that cells have highly irregular 3D shapes and exhibit numerous neighbour intercalations along the apical-basal axis as well as over time. Despite the fluidic nature, the cell packing configurations follow fundamental relationships previously described for apical epithelial layers, that is, Euler's polyhedron formula, Lewis' law, and Aboav-Weaire's law, at all times and across the entire tissue thickness. This arrangement minimises the lateral cell-cell surface energy for a given cross-sectional area variability, generated primarily by the distribution and movement of nuclei. We conclude that the complex 3D cell organisation in growing epithelia emerges from simple physical principles.
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