期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.73368
关键词
stress response; metabolic filaments; enzyme allostery; cryo-EM; S; cerevisiae
类别
资金
- US National Institutes of Health [R01 GM118396, S10 OD032290, T32 GM007270]
Many metabolic enzymes can self-assemble into filaments to organize and regulate metabolism, with this process often coinciding with significant metabolic changes. In yeast, cytoplasmic acidification upon starvation triggers the assembly of many metabolic enzymes into filaments, including CTPS. The assembly mechanism of CTPS is pH-sensitive and is conserved across different species.
Many metabolic enzymes self-assemble into micron-scale filaments to organize and regulate metabolism. The appearance of these assemblies often coincides with large metabolic changes as in development, cancer, and stress. Yeast undergo cytoplasmic acidification upon starvation, triggering the assembly of many metabolic enzymes into filaments. However, it is unclear how these filaments assemble at the molecular level and what their role is in the yeast starvation response. CTP Synthase (CTPS) assembles into metabolic filaments across many species. Here, we characterize in vitro polymerization and investigate in vivo consequences of CTPS assembly in yeast. Cryo-EM structures reveal a pH-sensitive assembly mechanism and highly ordered filament bundles that stabilize an inactive state of the enzyme, features unique to yeast CTPS. Disruption of filaments in cells with non-assembly or pH-insensitive mutations decreases growth rate, reflecting the importance of regulated CTPS filament assembly in homeotstasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据