期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.69019
关键词
Cupriavidus necator; Ralstonia eutropha; resource balance analysis; substrate limitation; co2 fixation; gene fitness; Other
类别
资金
- Swedish Research Council Vetenskapsradet [2016-06160]
- Swedish Research Council Formas [2015-939, 2019-01491]
- Novo Nordisk Fonden [NNF20OC0061469]
- Novo Nordisk Fonden [NNF20OC0061469] Funding Source: researchfish
- Forte [2019-01491] Funding Source: Forte
- Formas [2019-01491] Funding Source: Formas
The study revealed that the 'knallgas' bacterium reallocates protein resources when grown on different limiting substrates, with a large fraction of proteome invested in functions that are hardly utilized. The findings suggest that the bacterial growth strategy involves investing in readiness for future utilization, rather than immediate benefits.
Bacteria must balance the different needs for substrate assimilation, growth functions, and resilience in order to thrive in their environment. Of all cellular macromolecules, the bacterial proteome is by far the most important resource and its size is limited. Here, we investigated how the highly versatile 'knallgas' bacterium Cupriavidus necator reallocates protein resources when grown on different limiting substrates and with different growth rates. We determined protein quantity by mass spectrometry and estimated enzyme utilization by resource balance analysis modeling. We found that C. necator invests a large fraction of its proteome in functions that are hardly utilized. Of the enzymes that are utilized, many are present in excess abundance. One prominent example is the strong expression of CBB cycle genes such as Rubisco during growth on fructose. Modeling and mutant competition experiments suggest that CO2-reassimilation through Rubisco does not provide a fitness benefit for heterotrophic growth, but is rather an investment in readiness for autotrophy.
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