期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.72833
关键词
cell cycle; spindle position checkpoint; PP1; signalling; mitotic exit; S; cerevisiae
类别
资金
- EMBO [IG - 3918]
- TUBITAK [117C041, 118Z979, 117Z232]
- European Commission H2020-MSCA-IF [796599]
- ICGEB [CRP/TUR17-04_EC]
- Marie Curie Actions (MSCA) [796599] Funding Source: Marie Curie Actions (MSCA)
In this study, a novel checkpoint component Glc7-Bud14 was identified, which promotes dephosphorylation of the SPOC effector protein Bfa1. The results suggest that two mechanisms act in parallel for a robust checkpoint response, where SPOC kinase Kin4 isolates Bfa1 away from inhibitory kinase Cdc5, and Glc7-Bud14 dephosphorylates Bfa1 to fully activate the checkpoint effector.
Mitotic exit in budding yeast is dependent on correct orientation of the mitotic spindle along the cell polarity axis. When accurate positioning of the spindle fails, a surveillance mechanism named the spindle position checkpoint (SPOC) prevents cells from exiting mitosis. Mutants with a defective SPOC become multinucleated and lose their genomic integrity. Yet, a comprehensive understanding of the SPOC mechanism is missing. In this study, we identified the type 1 protein phosphatase, Glc7, in association with its regulatory protein Bud14 as a novel checkpoint component. We further showed that Glc7-Bud14 promotes dephosphorylation of the SPOC effector protein Bfa1. Our results suggest a model in which two mechanisms act in parallel for a robust checkpoint response: first, the SPOC kinase Kin4 isolates Bfa1 away from the inhibitory kinase Cdc5, and second, Glc7-Bud14 dephosphorylates Bfa1 to fully activate the checkpoint effector.
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