期刊
ELIFE
卷 11, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.73992
关键词
Ciona robusta; Xenopus; notochord; gene regulatory network; Brachyury; XBP1; C; intestinalis
类别
资金
- Eunice Kennedy Shriver National Institute of Child Health and Human Development of the National Institutes of Health [R03HD098395, R03HD098395-02S1]
- New York University Center for Skeletal and Craniofacial Biology by NIH [1P30DE020754]
- NIH [T32HD007520, T32GM008539]
- Cancer Center Support Grant NIH/NCI [P30CA016087]
Gene regulatory networks play a crucial role in coordinating the formation of organs and structures in different organisms. In this study, we investigated the regulatory interaction between Xbp1 and Brachyury in the development of the notochord. Our findings suggest that Brachyury and Xbp1 form a crucial subcircuit in the gene regulatory network of the notochord, which has been conserved during chordate evolution.
Gene regulatory networks coordinate the formation of organs and structures that compose the evolving body plans of different organisms. We are using a simple chordate model, the Ciona embryo, to investigate the essential gene regulatory network that orchestrates morphogenesis of the notochord, a structure necessary for the proper development of all chordate embryos. Although numerous transcription factors expressed in the notochord have been identified in different chordates, several of them remain to be positioned within a regulatory framework. Here, we focus on Xbp1, a transcription factor expressed during notochord formation in Ciona and other chordates. Through the identification of Xbp1-downstream notochord genes in Ciona, we found evidence of the early co-option of genes involved in the unfolded protein response to the notochord developmental program. We report the regulatory interplay between Xbp1 and Brachyury, and by extending these results to Xenopus, we show that Brachyury and Xbp1 form a cross-regulatory subcircuit of the notochord gene regulatory network that has been consolidated during chordate evolution.
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