期刊
ELIFE
卷 10, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.68224
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资金
- Shanghai basic research field Project [18JC1410100]
- Shanghai Municipal Science and Technology Major Project [2018SHZDZX05]
- National Key Research and Development Program of China [2020YFA0112700]
- Strategic Priority Research Program of Chinese Academy of Science [XDB32000000]
- National Natural Science Foundation of China [3187060071]
- State Key Laboratory of Neuroscience
Researchers generated single-cell transcriptomes of cells in different regions of larval zebrafish brains, confirming major brain cell types. Neuronal clusters with similar features often showed different transcription factor profiles, while neurotransmitter-type sister clusters predominantly expressed the same profiles.
Multidimensional landscapes of regulatory genes in neuronal phenotypes at whole-brain levels in the vertebrate remain elusive. We generated single-cell transcriptomes of similar to 67,000 region- and neurotransmitter/neuromodulator-identifiable cells from larval zebrafish brains. Hierarchical clustering based on effector gene profiles ('terminal features') distinguished major brain cell types. Sister clusters at hierarchical termini displayed similar terminal features. It was further verified by a population-level statistical method. Intriguingly, glutamatergic/GABAergic sister clusters mostly expressed distinct transcription factor (TF) profiles ('convergent pattern'), whereas neuromodulator-type sister clusters predominantly expressed the same TF profiles ('matched pattern'). Interestingly, glutamatergic/GABAergic clusters with similar TF profiles could also display different terminal features ('divergent pattern'). It led us to identify a library of RNA-binding proteins that differentially marked divergent pair clusters, suggesting the post-transcriptional regulation of neuron diversification. Thus, our findings reveal multidimensional landscapes of transcriptional and post-transcriptional regulators in whole-brain neuronal phenotypes in the zebrafish brain.
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