期刊
CHEMISTRYOPEN
卷 10, 期 12, 页码 1170-1176出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/open.202100222
关键词
cancer; drug delivery; iproplatin; liposome; ultrasound
资金
- Wellcome Trust [201406/Z/16/Z]
- John Fell Fund
- Children with Cancer UK [16-224]
- Cancer Research UK through the Cancer Research UK Oxford Centre [C5255/A18085]
- Wellcome Trust [201406/Z/16/Z] Funding Source: researchfish
- Wellcome Trust [201406/Z/16/Z] Funding Source: Wellcome Trust
Active loading of the Pt-IV prodrug iproplatin into liposomes using a calcium acetate gradient resulted in a 3-fold enhancement in drug concentration compared to passive loading strategies. Ultrasound-triggered release of iproplatin from microbubble-liposome construct offers greater control over drug delivery, leading to increased platinum concentration in breast cancer cells.
The Pt-IV prodrug iproplatin has been actively loaded into liposomes using a calcium acetate gradient, achieving a 3-fold enhancement in drug concentration compared to passive loading strategies. A strain-promoted cycloaddition reaction (azide- dibenzocyclooctyne) was used to attach iproplatin-loaded liposomes L(Pt) to gas-filled microbubbles (M), forming an ultrasound-responsive drug delivery vehicle [M-L(Pt)]. Ultrasound-triggered release of iproplatin from the microbubble-liposome construct was evaluated in cellulo. Breast cancer (MCF-7) cells treated with both free iproplatin and iproplatin-loaded liposome-microbubbles [M-L(Pt)] demonstrated an increase in platinum concentration when exposed to ultrasound. No appreciable platinum uptake was observed in MCF-7 cells following treatment with L(Pt) only or L(Pt)+ultrasound, suggesting that microbubble-mediated ultrasonic release of platinum-based drugs from liposomal carriers enables greater control over drug delivery.
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