Screening of Parkinson's Differential MicroRNA Based on GEO Database and Its Clinical Verification

Objective. This study is set out to explore the potential difference of miR in PD through GEO data and provide diagnostic indicators for clinical practice. Methods. In this study, differential miR was screened through the Gene Expression Omnibus (GEO) database, 68 PD patients treated in our hospital from May 2017 to March 2018 were collected as the research group (RG), and 50 normal subjects who underwent physical examination in our hospital during the same period were collected as the control group (CG). Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression and diagnostic value of miR-374a-5p in serum of patients. The potential target genes of miR-374a-5p were predicted, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Gene Ontology Consortium (GO) were carried out. Results. GEO2R analysis revealed that 193 miRs are expressed differentially, of which 78 were highly expressed and 115 were poorly expressed. The miR-374a-5p expression in the serum of the RG was reduced markedly and had a diagnostic value. Targetscan and miRDB online websites were used to predict their target genes, with 415 common target genes. miR-374a-5p may participate in 27 functional pathways and 8 signal pathways. Conclusion. miR-335-5p has low expression in PD and is expected to be a potential diagnostic indicator.

Automated summary

This study explored the potential difference of miR in PD using GEO data, and found that miR-374a-5p expression was significantly reduced in PD patients and had diagnostic value. The miR-374a-5p may participate in multiple functional pathways.