4.7 Article

Chloroplast morphology and pyrenoid ultrastructural analyses reappraise the diversity of the lichen phycobiont genus Trebouxia (Chlorophyta)

出版社

ELSEVIER
DOI: 10.1016/j.algal.2021.102561

关键词

Axenic culture; Microalgae; Microscopy; Species-level lineage; Symbiosis

资金

  1. Prometeo Excellence in Research Program (Generalitat Valenciana, Spain) [PROMETEO/2017/039, PROMETEO/2021/005]
  2. Valencian Government
  3. European Social Fund [APOSTD19]

向作者/读者索取更多资源

Trebouxiophyceae is a diverse class of green algae found in terrestrial and aquatic environments. Among them, the genus Trebouxia is widely distributed in lichens worldwide. However, the current species descriptions do not fully represent the overall diversity of the genus. By studying the ultrastructure of pyrenoids and the morphology of chloroplasts, it is possible to differentiate and characterize species-level lineages effectively.
Trebouxiophyceae is a wide class of green algae comprising coccoid and elliptic unicells, filaments, blades and colony-forming species that occur in diverse terrestrial and aquatic environments. Within this class, the genus Trebouxia Puymaly is among the most widespread lichen phycobionts worldwide. However, the 29 formally described species based on the combination of morphological traits and genetic diversity woefully underrepresented the overall species-level diversity recognized in the genus. In Trebouxia, reliable differentiation and characterization of the species-level lineages can be achieved by studying the diversity of key diagnostic features of pyrenoid ultrastructure and chloroplast morphology of axenically grown algal cultures. Here, we used transmission electron microscopy (TEM) coupled with confocal laser scanning microscopy (CLSM) to analyze the pyrenoid and the chloroplast of 20 Trebouxia species-level lineages grown directly on solid agar medium and on cellulose-acetate discs laid over the agar medium. With the new, detailed morphoanatomical characterization of these species-level lineages, we reappraise Trebouxia taxonomy in light of the most recent phylogenetic delimitation provided by Muggia et al. (2020).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据