期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 60, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.algal.2021.102512
关键词
Macroalgae; Alginate lyase; Seaweed saccharification; Bacillus; Seaweed biorefining
资金
- Norwegian research council project The Norwegian Seaweed Biorefinery Platform [294946]
- China Scholarship Council
This study demonstrates that using culture supernatants directly as a source of alginate lyases is an efficient method for saccharification of seaweed biomass, improving the glucose concentration by 73% compared to using only cellulases.
Brown seaweeds are rich in carbohydrates and may be used as a source of fermentable sugars. Saccharification of the seaweed biomass can be carried out enzymatically by a combination of cellulases and alginate lyases. In this study, thermotolerant exo- and endo alginate lyases were cloned and expressed in Bacillus subtilis. The lyases were secreted to the culture supernatant and used directly together with a commercial cellulase preparation to saccharify Saccharina latissima biomass. The results showed that the strategy of using the culture supernatants directly as a source of alginate lyases worked very well, releasing glucose, mannitol, and uronic acids. The ratio between the exo- and endo-acting alginate lyases proved to be very important for saccharification yield, and under optimal reaction conditions the use of culture supernatants containing alginate lyases improved final glucose concentration by 73%, when compared to only applying cellulases. This direct use of culture supernatants as a source of alginate lyases shows that enzyme purification steps are not needed, saving seaweed processing costs and points to the possibility of a relatively simple on-site enzyme production for seaweed biorefining.
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