期刊
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
卷 10, 期 9, 页码 2881-2889出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.1c07336
关键词
geranyl acetate; geraniol; co-expression; fermentation; Escherichia coli
资金
- National Natural Science Foundation of China [21878160]
- Top-notch Academic Programs Project (TAPP) of Jiangsu Higher Education Institutions
- Open Foundation of Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration [JPELBCPI2016004]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX21_0897]
This study reports a microbial cell factory that achieves selective and high-level production of geranyl acetate in Escherichia coli. The combination of genetic and bioprocess engineering provides a promising strategy for improving yield and selectivity, with potential applications in the synthesis of other monoterpene esters in E. coli.
Geranyl acetate is, as one of the monoterpenoids, a natural constituent of more than 60 essential oils. Production of such an ester fragrance compound by plant extracts is limited by low yield and high processing costs but plausible by microbial synthesis. We report a microbial cell factory that realizes selective and high-level geranyl acetate production in Escherichia coli. For this purpose, co-expression of geraniol synthase and alcohol acetyltransferase was initially used to increase production yield, but a considerable quantity of precursor geraniol was also produced. Further, introducing an extra AAT gene copy and controlling the substrate glycerol supply enabled a drastically higher selective geranyl acetate production. Eventually, the highest reported titer of 52.78 mM (equivalent to 10.36 g/L) geranyl acetate, accounting for 98.5% of total products, was achieved under a controlled fermentation system. Such selective and high-level geranyl acetate production by combining genetic and bioprocess engineering is also a promising strategy for other monoterpene esters in E. coli.
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