期刊
TOXINS
卷 14, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/toxins14020073
关键词
mycotoxins; antibodies; aptamers; short peptides; molecularly imprinted polymers; rapid tests; lateral flow assay; microplate assay; biosensor; multiplexing
资金
- National Natural Science Foundation of China [31501560]
- China Postdoctoral Science Foundation [2019M653767]
This review discusses the recent progress in the development of reliable bioreceptor-based analytical methods for mycotoxins by generating native biomolecules and new bioinspired materials. It presents an overview of different biorecognition molecules and their applications, as well as highlighting the commercial availability and future challenges in this field.
Mycotoxins are secondary metabolites produced by fungal species, which pose significant risk to humans and livestock. The mycotoxins which are produced from Aspergillus, Penicillium, and Fusarium are considered most important and therefore regulated in food- and feedstuffs. Analyses are predominantly performed by official laboratory methods in centralized labs by expert technicians. There is an urgent demand for new low-cost, easy-to-use, and portable analytical devices for rapid on-site determination. Most significant advances were realized in the field bioanalytical techniques based on molecular recognition. This review aims to discuss recent progress in the generation of native biomolecules and new bioinspired materials towards mycotoxins for the development of reliable bioreceptor-based analytical methods. After brief presentation of basic knowledge regarding characteristics of most important mycotoxins, the generation, benefits, and limitations of present and emerging biorecognition molecules, such as polyclonal (pAb), monoclonal (mAb), recombinant antibodies (rAb), aptamers, short peptides, and molecularly imprinted polymers (MIPs), are discussed. Hereinafter, the use of binders in different areas of application, including sample preparation, microplate- and tube-based assays, lateral flow devices, and biosensors, is highlighted. Special focus, on a global scale, is placed on commercial availability of single receptor molecules, test-kits, and biosensor platforms using multiplexed bead-based suspension assays and planar biochip arrays. Future outlook is given with special emphasis on new challenges, such as increasing use of rAb based on synthetic and naive antibody libraries to renounce animal immunization, multiple-analyte test-kits and high-throughput multiplexing, and determination of masked mycotoxins, including stereoisomeric degradation products.
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