4.7 Article

Development of an Efficient Extraction Method for Harvesting Gymnodimine-A from Large-Scale Cultures of Karenia selliformis

期刊

TOXINS
卷 13, 期 11, 页码 -

出版社

MDPI
DOI: 10.3390/toxins13110793

关键词

Karenia selliformis; gymnodimine; liquid-liquid extraction (LLE); extraction method

资金

  1. National Key R&D Program of China [2019YFC0312602]
  2. National Natural Science Foundation of China [41876112]
  3. INTERREG Atlantic Area ALERTOX-NET project [EAPA_317/2016]

向作者/读者索取更多资源

This study compared various liquid-liquid extraction (LLE) treatments to optimize the harvesting of GYM-A from Karenia selliformis cultures, highlighting the effectiveness of LLE over filtration and centrifugation methods. It was found that GYM-A degraded significantly in alkaline culture media but remained stable in acidic conditions, demonstrating the importance of pH in toxin stability. The developed LLE method allows for efficient extraction of GYM-A from large-volume cultures, providing a valuable technique for harvesting toxins from GYM-producing microalgae.
Gymnodimine-A (GYM-A) is a fast-acting microalgal toxin and its production of certified materials requires an efficient harvesting technology from the large-scale cultures of toxigenic microalgae. In this study the recoveries of GYM-A were compared between several liquid-liquid extraction (LLE) treatments including solvents, ratios and stirring times to optimize the LLE technique for harvesting GYM-A from Karenia selliformis cultures, of which the dichloromethane was selected as the extractant and added to microalgal cultures at the ratio 55 mL L-1 (5.5%, v/v). The recovery of GYM-A obtained by the LLE technique was also compared with filtration and centrifugation methods. The stability of GYM-A in culture media were also tested under different pH conditions. Results showed that both the conventional filter filtration and centrifugation methods led to fragmentation of microalgal cells and loss of GYM-A in the harvesting processes. A total of 5.1 mu g of GYM-A were obtained from 2 L of K. selliformis cultures with a satisfactory recovery of 88%. Interestingly, GYM-A obviously degraded in the culture media with the initial pH 8.2 and the adjusted pH of 7.0 after 7 days, but there was no obvious degradation in the acidic medium at pH 5.0. Therefore, the LLE method developed here permits the collection of large-volume cultures of K. selliformis and the high-efficiency extraction of GYM-A. This work provides a simple and valuable technique for harvesting toxins from large-scale cultures of GYM-producing microalgae.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据