期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 93, 期 -, 页码 136-144出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.05.100
关键词
Lentinus edodes polysaccharides; Enzyme-assisted extraction; Anti- proliferative activity
资金
- Hebei Administration of Traditional Chinese Medicine [2015173]
- Hebei Education Department
- Science and Technology Research Project of Hebei Higher School [ZD2015131, QN2016106]
- Hebei Education Department [QN2015211, Z2012014]
In this study, the enzyme-assisted extraction of polysaccharides from Lentinus edodes (LEPs) was optimized by response surface methodology, and a preliminary characterization of the extracted LEPs and their anti-proliferative activities were investigated. An orthogonal assay was constructed to determine the optimal amounts of cellulase, papain and pectinase, which were 15, 20 and 15 g/kg, respectively. Then effects of extraction conditions were evaluated and optimized using a Box-Behnken design. The results showed that the highest polysaccharides yield of 15.65% was achieved with an extraction temperature of 54 degrees C, pH 5.0, enzymatic treatment time of 93 min and a liquid/material ratio of 29:1 mL/g, which correlated well with the predicted yield of 15.58%. Subsequently, the crude LEPs were further purified by DEAE-cellulose and Sephadex-100 chromatography to obtain two fractions, which were designated as LEP-1 and LEP-2 and their monosaccharide compositions were characterized by GC. Fourier-transform infrared spectra demonstrated that LEP-1 and LEP-2 were distinct from each other regarding their chemical structures. In addition, the LEPs exhibited inhibition of cell proliferation on HCT-116 and HeLa cells in vitro. In summary, this study provides an efficient enzyme-assisted extraction for LEPs, which can be used as natural antitumor agents in the pharmaceutical and functional food industries. (C) 2016 Elsevier B.V. All rights reserved.
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