Effects of Ganoderma lucidum polysaccharides on chronic pancreatitis and intestinal microbiota in mice

This study manifested the effects of polysaccharides from Ganoderma lucidum strain S-3 (GLP S-3) on chronic pancreatitis (CP) therapy and intestinal microbiota modulation in mice induced by diethyldithiocarbamate (DDC). The GLPS(3) was prepared from cultured mycelium and markedly alleviated the pancreatitis in mice through decreasing lipase, AMS, IFN-1, and TNF-alpha level as well as increasing SOD and total antioxidant activity. Furthermore, high throughput sequencing analysis revealed that GLPS(3) altered the composition and diversity of intestinal microbiota, especially, decreased the relative abundance of phylum Bacteroidetes and increased that of phylum Firmictutes. At the genus level, supplementation of GLPS(3) increased the relative abundance of the beneficial bacteria such as Lactobacillales, Roseburia and Lachnospiraceae. These results disclosed the potential therapy mechanism of GLPS(3) on chronic pancreatitis might be intestinal microbiota dependent. (C) 2016 Elsevier B.V. All rights reserved.