Cytotoxic effect of fucoidan extracted from Sargassum cinereum on colon cancer cell line HCT-15

The present study was aimed to investigate the antioxidant and cytotoxicity activity against HCT-15 of fucoidan from Sargassum cinereum. Purification of fucoidan was done by DEAE cellulose and dialysis. Physicochemical characterization of fucoidan was analysed by calorimetric assay, FT-IR, HPLC and NMR. The extracted fucoidan contains 65.753% of fucose and 3.7 +/- 1.54% of sulphate respectively. HPLC results showed that the fucoidan contains the monosaccharide composition such as fucose, galactose, mannose and xylose. Antioxidant effect of fucoidan in Sargassum Cinereum was determined by DPPH. The maximum DPPH activity was found at the concentration of 100 mu g, where as the crude extract showed the scavenging activity was 63.58 +/- 0.56%. Cytotoxicity effect was done by MIT assay. Fucoidan extract caused about 50% of cell death after 24 h of incubation with 75 +/- 0.9037 mu g/ml against HCT-15. (C) 2016 Elsevier B.V. All rights reserved.