期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 91, 期 -, 页码 716-723出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.06.015
关键词
CMCS/CS-NPs; ECPs; Oral immunization; Adaptive and innate immune responses
资金
- National Natural Science Foundation of China [31500807]
- Public Science and Technology Research Founds Projects of Ocean [2015418022]
- National Natural Science Foundation of China NSFC-Shandong joint fund [U1406402-5]
- National Science Foundation [2014M560579]
- Fundamental Research Funds for the Central Universities [201513009]
- Applied Basic Research Plan of Qingdao [15-9-1-73-jch]
This study aimed to investigate the efficacy of nanoparticles based on chitosan as a vehicle for oral antigen delivery in fish vaccination. Carboxymethyl chitosan/chitosan nanoparticles (CMCS/CS-NPs) loaded extracellular products (ECPs) of Vibrio anguillarum were successfully developed by ionic gelation method. The prepared ECPs-loaded CMCS/CS-NPs were characterized for various parameters including morphology, particle size (312 +/- 7.18 nm), zeta potential (+17.4 +/- 0.38 mV), loading efficiency (57.8 +/- 2.54%) and stability under the simulated gastrointestinal (GI) tract conditions in turbot. The in vitro profile showed that the cumulative release of ECPs from nanoparticles was higher in pH 7.4 (58%) than in pH 2.0 (37%) and pH 4.5 (29%) after 48 h. Fluorescein isothiocyanate-labeled bovine serum albumin (FITC-BSA) was used as model protein antigen and encapsulated in CMCS/CS-NPs for investigating the biodistribution of antigen after oral delivery to turbot in 24 h. Oral immunization of ECPs-loaded CMCS/CS-NPs group in turbot showed elevated specific antibody and higher concentrations of lysozyme activity and complement activity in fish serum than ECPs solution. CMCS/CS-NPs loaded with ECPs could enhance both adaptive and innate immune responses than the group treated with ECPs solution and suggested to be a potential antigen delivery system. (C) 2016 Published by Elsevier B.V.
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