Biological Behavior of Xenogenic Scaffolds in Alcohol-Induced Rats: Histomorphometric and Picrosirius Red Staining Analysis

In this experimental protocol, the objective was to evaluate the biological behavior of two xenogenic scaffolds in alcohol-induced rats through histomorphometric and Picrosirius Red staining analysis of non-critical defects in the tibia of rats submitted or not to alcohol ingestion at 25% v/v. Eighty male rats were randomly divided into four groups (n = 20 each): CG/B (water diet + Bio-Oss((R)) graft, Geistlich Pharma AG, Wolhusen, Switzerland), CG/O (water diet + OrthoGen((R)) graft, Baumer, Mogi Mirim, Brazil), AG/B (25% v/v alcohol diet + Bio-Oss((R)) graft), and AG/O (25% v/v alcohol diet + OrthoGen((R)) graft). After 90 days of liquid diet, the rats were surgically obtained, with a defect in the tibia proximal epiphysis; filled in according to their respective groups; and euthanized at 10, 20, 40 and 60 days. In two initial periods (10 and 20 days), all groups presented biomaterial particles surrounded by disorganized collagen fibrils. Alcoholic animals (AG/B and AG/O) presented, in the cortical and medullary regions, a reactive tissue with inflammatory infiltrate. In 60 days, in the superficial area of the surgical cavities, particles of biomaterials were observed in all groups, with new compact bone tissue around them, without complete closure of the lesion, except in non-alcoholic animals treated with Bio-Oss((R)) xenograft (CG/B), where the new cortical interconnected the edges of the defect. Birefringence transition was observed in the histochemical analysis of collagen fibers by Picrosirius Red, in which all groups in periods of 10 and 20 days showed red-orange birefringence, and from 40 days onwards greenish-yellow birefringence, which demonstrates the characteristic transition from the formation of thin and disorganized collagen fibers initially to more organized and thicker later. In histomorphometric analysis, at 60 days, CG/B had the highest volume density of new bone (32.9 +/- 1.15) and AG/O the lowest volume density of new bone (15.32 +/- 1.71). It can be concluded that the bone neoformation occurred in the defects that received the two biomaterials, in all periods, but the Bio-Oss((R)) was superior in the results, with its groups CG/B and AG/B displaying greater bone formation (32.9 +/- 1.15 and 22.74 +/- 1.15, respectively) compared to the OrthoGen((R)) CG/O and AG/O groups (20.66 +/- 2.12 and 15.32 +/- 1.71, respectively), and that the alcoholic diet interfered negatively in the repair process and in the percentage of new bone formed.

Automated summary

The experiment aimed to evaluate the biological behavior of two xenogenic scaffolds in alcohol-induced rats with tibia defects. Results showed that both biomaterials promoted bone formation, with Bio-Oss((R)) being superior to OrthoGen((R)), while alcohol intake had a negative impact on the healing process and new bone formation.