Self-Assembly of Soluble Chitosan Derivatives Nanoparticles for Vaccine: Synthesis, Characterization and Evaluation

Herein, a novel chitosan derivative nanoparticle was proposed to function as a delivery carrier. First of all, an improvement was made to the way N-2-hydroxypropyl trimcthyl ammonium chloride chitosan (N-2-HACC) was synthesized. Moreover, the solution to one-step synthesis of N-2-HACC from chitosan (CS) was developed. Different from the previous report, the synthesis process was simplified, and there was a reduction in the amount of 2,3-epoxypropyl trimethyl ammonium chloride (EPTAC) used. With its excellent water solubility maintained, the relatively low degree of substitution was controlled to facilitate the cross-linking reaction. The results obtained from H-1-NMR, FTIR spectroscopy, and XRD indicated a smooth EPTAC onto CS for the formation of N-2-HACC with 59.33% the degree of substitution (DS). According to our results, N-2-HACC could be dissolved in various organic solvents, deionized water, 1% acetic acid aqueous solution, and others at room temperature. Finally, a novel chitosan nanoparticle material was prepared using the self-assembly method with beta-glycerophosphate sodium (beta-GC), with excellent immune properties achieved, thus providing a new strategy for chitosan self-assembled nanoparticles.

Automated summary

A novel chitosan derivative nanoparticle was proposed for delivery purposes, with an improved synthesis method for N-2-HACC and a one-step synthesis solution from chitosan developed. By simplifying the synthesis process and reducing the amount of EPTAC used, a relatively low degree of substitution was controlled for cross-linking reactions, leading to the formation of N-2-HACC. The self-assembly preparation of a novel chitosan nanoparticle material with beta-GC resulted in excellent immune properties, offering a new strategy for chitosan self-assembled nanoparticles.