期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 82, 期 -, 页码 607-613出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2015.10.083
关键词
Se-enriched Grifola frondosa; Polysaccharide; Characterization; Anti-tumor; Immunomodulatory
资金
- Public Welfare Technology Application Research Project in Zhejiang Province [2012C22008]
- Graduate Innovative Projects in Jiangsu Province [CXZZ11-0601]
- Undergraduate Innovative Training Project in Jiangsu Province [201410299019Z]
- Senior Talent Start-up Fund in Jiangsu University [15JDG031]
A polysaccharide termed Se-GP11 was extracted and purified from Se-enriched Grifola frondosa in our previous study. This study investigated the characterization, anti-tumor and immunomodulatory activity of Se-GP11. The results showed that Se-GP11 was composed of mannose, glucose and galactose with a molar ratio of 1:4.91:2.41. The weight-average molecular weight (M-w) and weight-average mean square radius (R-w) of Se-GP11 in 0.1 M sodium chloride solution were 3.3 x 10(4) Da and 32.8 nm. Se-GP11 existed as a globular conformation with random coil structure. Se-GP11 had no anti-tumor activity against HepG-2 cells in vitro, and it significantly inhibited the growth of Heps tumor in vivo. Se-GP11 increased the relatively thymus and spleen weights as well as serum necrosis factor-alpha (TNF-alpha) and interleukin-2 (IL-2) levels. In addition, Se-GP11 promoted the phagocytosis and NO production of RAW264.7 as compared with that of the normal control group. The results revealed that the Se-GP11 may exhibit the anti-tumor through improving immunologic function of the tumor bearing mice. (c) 2015 Elsevier B.V. All rights reserved.
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