Rehardening and the Protective Effect of Gamma-Polyglutamic Acid/Nano-Hydroxyapatite Paste on Surface-Etched Enamel

Many revolutionary approaches are on the way pertaining to the high occurrence of tooth decay, which is an enduring challenge in the field of preventive dentistry. However, an ideal dental care material has yet to be fully developed. With this aim, this research reports a dramatic enhancement in the rehardening potential of surface-etched enamels through a plausible synergistic effect of the novel combination of gamma-polyglutamic acid (gamma-PGA) and nano-hydroxyapatite (nano-HAp) paste, within the limitations of the study. The percentage of recovery of the surface microhardness (SMHR%) and the surface parameters for 9 wt% gamma-PGA/nano-HAp paste on acid-etched enamel were investigated with a Vickers microhardness tester and an atomic force microscope, respectively. This in vitro study demonstrates that gamma-PGA/nano-HAp treatment could increase the SMHR% of etched enamel to 39.59 & PLUSMN; 6.69% in 30 min. To test the hypothesis of the rehardening mechanism and the preventive effect of the gamma-PGA/nano-HAp paste, the surface parameters of mean peak spacing (Rsm) and mean arithmetic surface roughness (Ra) were both measured and compared to the specimens subjected to demineralization and/or remineralization. After the treatment of gamma-PGA/nano-HAp on the etched surface, the reduction in Rsm from 999 & PLUSMN; 120 nm to 700 & PLUSMN; 80 nm suggests the possible mechanism of void-filling within a short treatment time of 10 min. Furthermore, & UDelta;Ra-I, the roughness change due to etching before remineralization, was 23.15 & PLUSMN; 3.23 nm, while & UDelta;Ra-II, the roughness change after remineralization, was 11.99 & PLUSMN; 3.90 nm. This statistically significant reduction in roughness change (p < 0.05) implies a protective effect against the demineralization process. The as-developed novel gamma-PGA/nano-HAp paste possesses a high efficacy towards tooth microhardness rehardening, and a protective effect against acid etching.

Automated summary

This study demonstrates a significant enhancement in the rehardening potential of surface-etched enamels through a novel combination of γ-PGA and nano-HAp, showing promising preventive effects against tooth decay.