4.4 Article

Conditional deletion of ROCK2 induces anxiety-like behaviors and alters dendritic spine density and morphology on CA1 pyramidal neurons

期刊

MOLECULAR BRAIN
卷 14, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s13041-021-00878-4

关键词

ROCK2; Rho kinase; Dendritic spine; Hippocampus; Prefrontal cortex; Amygdala

资金

  1. National Institutes of Health [NINDS T32 NS095775, NINDS T32 NS061788, NIA F31 AG067635]
  2. NIA [F31 AG067635, AG061800, AG054719, AG063755]

向作者/读者索取更多资源

The researchers generated ROCK2(fl/fl) mice and found that Cre/ROCK2(fl/fl) mice exhibited anxiety-like behaviors. Different spine density and morphology were observed in CA1 hippocampus, mPFC, and BLA among the mice.
Rho-associated kinase isoform 2 (ROCK2) is an attractive drug target for several neurologic disorders. A critical barrier to ROCK2-based research and therapeutics is the lack of a mouse model that enables investigation of ROCK2 with spatial and temporal control of gene expression. To overcome this, we generated ROCK2(fl/fl) mice. Mice expressing Cre recombinase in forebrain excitatory neurons (CaMKII-Cre) were crossed with ROCK2(fl/fl) mice (Cre/ROCK2(fl/fl)), and the contribution of ROCK2 in behavior as well as dendritic spine morphology in the hippocampus, medial prefrontal cortex (mPFC), and basolateral amygdala (BLA) was examined. Cre/ROCK2(fl/fl) mice spent reduced time in the open arms of the elevated plus maze and increased time in the dark of the light-dark box test compared to littermate controls. These results indicated that Cre/ROCK2(fl/fl) mice exhibited anxiety-like behaviors. To examine dendritic spine morphology, individual pyramidal neurons in CA1 hippocampus, mPFC, and the BLA were targeted for iontophoretic microinjection of fluorescent dye, followed by high-resolution confocal microscopy and neuronal 3D reconstructions for morphometry analysis. In dorsal CA1, Cre/ROCK2(fl/fl) mice displayed significantly increased thin spine density on basal dendrites and reduced mean spine head volume across all spine types on apical dendrites. In ventral CA1, Cre/ROCK2(fl/fl) mice exhibited significantly increased spine length on apical dendrites. Spine density and morphology were comparable in the mPFC and BLA between both genotypes. These findings suggest that neuronal ROCK2 mediates spine density and morphology in a compartmentalized manner among CA1 pyramidal cells, and that in the absence of ROCK2 these mechanisms may contribute to anxiety-like behaviors.

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