期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 93, 期 -, 页码 27-33出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.08.058
关键词
Cordyceps militaris; Acid hydrolysis; Inhibitory activity
资金
- National Spark Key Program of China [2015GA610001]
- Natural Science Foundation of Tianjin [16JCZDJC34100]
- International Science and Technology Cooperation Program of China [2013DFA31160]
The structural properties and the inhibition on alpha-D-glucosidase activity of the low molecular weight (LCMPs-II) obtained from the optimized acid hydrolysis of the Cordyceps militaris polysaccharides (CMPs) were investigated in this paper. The LCMPs-II with a molecular weight of 28 KDa mainly composed of rhamnose, xylose and glucose with the molar ratio of 1: 2.19: 6.73 was separated from LCMPs-I which was the acid hydrolysis product of CMPs by chromatography on Sephadex G-100 column. The solubility of LCMPs-II was tested to be 32.12 +/- 1.05 gin 100 mL distilled water under 25 degrees C. Its solubility was almost as twice as that of CMPs. Afterward, the structural features of LCMPs-II was investigated by a combination of chemical and instrumental analysis such as the specific rotation determination, FT-IR, periodate oxidation-Smith degradation, Congo-red, GC, scanning electron microscope and NMR. The results showed that the optical rotation of LCMPs-II was +25 degrees and it was 1,3-branched-rhamnoxyloglucan which had a linear backbone of (1 -> 4)-linked alpha-D-glucopyranose (alpha-D-Glcp units). (C) 2016 Elsevier B.V. All rights reserved.
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