Selection of the most effective kinetic model of lactase hydrolysis by immobilized Aspergillus niger and free β-galactosidase

The kinetic model of the hydrolysis of lactose by beta-galactosidase from Aspergillus niger immobilized on a commercial ceramic monoliths was estimated in the attendance of lactose and its hydrolysis reaction products galactose and glucose. The aim of this work was to developing kinetic model of lactase hydrolysis by Aspergillus niger. The variables in this study are temperature, pH, enzyme concentration, substrate concentration and final product. The optimum temperature used to achieve the best hydrolysis performance in the kinetic model selection was 55 and 60 degrees C. The optimum pH used for enzyme activity was about 3.5 to 4. Five kinetic models were proposed to confirm experimental data the enzymatic reaction of the lactose hydrolysis by the beta-galactosidase. The kinetics of lactose hydrolysis by both Immobilized and soluble lactases were scrutinized in a batch reactor system in the lack of any mass conduction restriction. In both instance the galactose inhibition kinetic models predicted the experimental data. The model is capable to fit the experimental data correctly in the extensive experimental span studied. (C) 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University.

Automated summary

The study developed a kinetic model of lactase hydrolysis by Aspergillus niger, considering variables such as temperature, pH, enzyme concentration, substrate concentration, and final product. Five kinetic models were proposed, with optimal temperature and pH ranges identified for enzyme activity. The models successfully predicted experimental data for lactose hydrolysis by beta-galactosidase.