Response of carbohydrate metabolism-mediated sink strength to auxin in shoot tips of apple plants

Auxin (indole-3-acetic acid, IAA) has a considerable impact on the regulation of plant carbohydrate levels and growth, but the mechanism by which it regulates sugar levels in plants has received little attention. In this study, we found that exogenous IAA altered fructose (Fru), glucose (Glc), and sucrose (Suc) concentrations in shoot tips mainly by regulating MdSUSY1, MdFRK2, MdHxK1 and MdSDH2 transcript levels. Additionally, we used 5-year-old 'Royal Gala' apple trees to further verify that these genes play primary roles in regulating sink strength. The results showed that MdSUSY1, MdFRK2, MdHxK1/3 and MdSDH2 might be major contributors to sink strength regulation. Taken together, these results provide new insight into the regulation of the carbohydrate metabolism mechanism, which will be helpful for regulating sink strength and yield.

Automated summary

This study found that auxin can alter sugar concentrations in plants by regulating the transcript levels of different genes, which in turn affects plant growth and carbohydrate levels. Specifically, in apple trees, MdSUSY1, MdFRK2, MdHxK1/3, and MdSDH2 genes play significant roles in regulating sink strength.