期刊
JOURNAL OF CENTRAL SOUTH UNIVERSITY
卷 28, 期 12, 页码 3642-3653出版社
JOURNAL OF CENTRAL SOUTH UNIV
DOI: 10.1007/s11771-021-4844-1
关键词
gold nanoparticles; action potential; voltage-gated sodium current; hippocampus; patch clamp
资金
- Natural Science Foundation of Zhejiang Province, China [LY19C090004]
- Natural Science Foundation of Jiangsu Province, China [BK20200710, BK2018077]
- Nanjing Polytechnic Institute Start Fund, China [NHKY-2019-19]
- Innovation and Entrepreneurship Training Program of Jiangsu Province College Students, China [202012920026Y]
The study showed that the shape of gold nanoparticles (GNPs) affects the voltage-dependent sodium channel and action potential of hippocampal CA1 neurons in mice. GNPs inhibited the sodium current amplitudes and shifted the voltage-dependence curve, leading to increased neuronal excitability and altered action potential properties. Nanoflower-shaped GNPs had the most significant effects on action potential properties, indicating that the neurotoxicity of GNPs is dependent on surface roughness.
Gold nanoparticles (GNPs) have been extensively used in nanomedicine and neuroscience owing to their biological inertness, peculiar opto-electronic and physico-chemical features. However, the effect of GNPs shape on the neurophysiological properties of single neuron is still unclear. To tackle this issue, different shape GNPs (nanosphere, nanotriakisoctahedron and nanoflower) were synthesized to investigate the effect of GNPs on the voltage-dependent sodium channel and the action potential (AP) of hippocampal CA1 neurons in mice. The results indicated that GNPs inhibited the amplitudes of voltage-gated sodium current (I-Na) and led to a hyperpolarizing shift in the voltage-dependence curve of both activation and inactivation of I-Na. GNPs also increased neuronal excitability and altered some properties of AP. Moreover, most alterations in AP properties were observed in nanoflower GNPs treated CA1 neurons, suggesting that the neurotoxicity of gold nanoparticles is surface roughness-dependent. These results may provide a valuable direction in the clinical application of GNPs.
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