期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
卷 87, 期 -, 页码 208-221出版社
ELSEVIER
DOI: 10.1016/j.ijbiomac.2016.02.040
关键词
alpha-Crystallin; Copper ion; Peroxynitrite; Ascorbic acid; Chaperone activity
资金
- Iran National Science Foundation (INSF) [92001695]
- research council of Shiraz University
The copper-catalyzed oxidation of ascorbic acid (ASA) to dehydroascorbate (DHA) and hydrogen peroxide plays a central role in pathology of cataract diseases during ageing and in diabetic patients. In the current study, the structural feature, chaperone-like activity and protective ability of peroxynitrite (PON) modified alpha A- and alpha B-Crystallin (Cry) against copper-mediated ASA oxidation were studied using different spectroscopic measurements and gel mobility shift assay. Upon PON modification, additional to protein structural alteration, the contents of nitrotyrosine, nitrotryptophan, dityrosine and carbonyl groups were significantly increased. Moreover, alpha B-Cry demonstrates significantly larger capacity for PON modification than alpha A-Cry. Also, based on the extent of PON modification, these proteins may display an improved chaperone-like activity and enhanced protective ability against copper-mediated ASA oxidation. In the presence of copper ions, chaperone-like activity of both native and PON-modified alpha-Cry subunits were appreciably improved. Additionally, binding of copper ions to native and PON-modified proteins results in the significant reduction of their solvent exposed hydrophobic patches. Overall, the increase in chaperone-like activity/ASA protective ability of PON-modified alpha-Cry and additional enhancement of its chaperoning action with copper ions appear to be an important defense mechanism offered by this protein. (C) 2016 Elsevier B.V. All rights reserved.
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