4.3 Article

A chromosomal-level reference genome of the widely utilized Coccidioides posadasii laboratory strain Silveira

期刊

G3-GENES GENOMES GENETICS
卷 12, 期 4, 页码 -

出版社

OXFORD UNIV PRESS INC
DOI: 10.1093/g3journal/jkac031

关键词

valley fever; long-read sequencing; funannotate; human fungal pathogen; fungal genomes; reference genome; coccidioidomycosis

资金

  1. Arizona Biomedical Research Commission/Arizona Department of Health Services [18-198851]
  2. National Institutes of Health/National Institute of Allergy and Infectious Disease [R21AI28536-01AI]
  3. University of California [MRP-17-454959, VFR-19-633952]
  4. United States Department of AgricultureNational Institute of Food and Agriculture Hatch Project [CA-RPPA-5062-H]

向作者/读者索取更多资源

This study sequenced and annotated the genome of the Coccidioides posadasii Silveira strain using high-quality sequencing technology, providing a foundation for future genetic studies and improving understanding of the chromosomal structure and function of this strain.
Coccidioidomycosis is a common fungal disease that is endemic to arid and semi-arid regions of both American continents. Coccidioides immitis and Coccidioides posadasii are the etiological agents of the disease, also known as Valley Fever. For several decades, the C. posadasii strain Silveira has been used widely in vaccine studies, is the source strain for production of diagnostic antigens, and is a widely used experimental strain for functional studies. In 2009, the genome was sequenced using Sanger sequencing technology, and a draft assembly and annotation were made available. In this study, the genome of the Silveira strain was sequenced using single molecule real-time sequencing PacBio technology, assembled into chromosomal-level contigs, genotyped, and the genome was reannotated using sophisticated and curated in silico tools. This high-quality genome sequencing effort has improved our understanding of chromosomal structure, gene set annotation, and lays the groundwork for identification of structural variants (e.g. transversions, translocations, and copy number variants), assessment of gene gain and loss, and comparison of transposable elements in future phylogenetic and population genomics studies.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据