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Seeing and touching adenovirus: complementary approaches for understanding assembly and disassembly of a complex virion

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CURRENT OPINION IN VIROLOGY
卷 52, 期 -, 页码 112-122

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ELSEVIER SCI LTD
DOI: 10.1016/j.coviro.2021.11.006

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资金

  1. Spanish Ministry of Economy, Industry and Competitiveness [FIS2017-89549-R]
  2. 'Maria de Maeztu' Program for Units of Excellence in RD [MDM-2014-0377, FIS2017-90701-REDT]
  3. Human Frontiers Science Program [HFSPO RGP0012/2018]
  4. Spanish State Research Agency
  5. European Regional Development Fund
  6. Spanish State Research Agency Severo Ochoa Excellence grant [SEV 2017-0712]
  7. CSIC funded consortium LifeHub [202120E47]
  8. Comunidad Autonoma de Madrid
  9. [PID2019-104098GB-I00/AEI/10.13039/501100011033]

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Understanding the assembly and disassembly of adenoviruses is challenging due to their complexity. Cryoelectron microscopy and atomic force microscopy have provided insights into the structure and function of adenoviruses, particularly the roles of core proteins.
Understanding adenovirus assembly and disassembly poses many challenges due to the virion complexity. A distinctive feature of adenoviruses is the large amount of virus-encoded proteins packed together with the dsDNA genome. Cryoelectron microscopy (cryo-EM) structures are broadening our understanding of capsid variability along evolution, but little is known about the organization of the non-icosahedral nucleoproteic core and its influence in adenovirus function. Atomic force microscopy (AFM) probes the biomechanics of virus particles, while simultaneously inducing and monitoring their disassembly in real time. Synergistic combination of AFM with EM shows that core proteins play unexpected key roles in maturation and entry, and uncoating dynamics are finely tuned to ensure genome release at the appropriate time and place for successful infection.

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