期刊
CELL AND BIOSCIENCE
卷 11, 期 1, 页码 -出版社
BMC
DOI: 10.1186/s13578-021-00718-x
关键词
Elastin remodeling; Elastin peptides; Elastin receptor complex; NEU1; Desialylation; Membrane glycoproteins; Monocytes; Endothelial cells
资金
- ANR [ANR-18-CE44-0017]
- CNRS
- URCA
- Region Grand Est
The study reveals that elastin-derived peptides interact with the elastin receptor complex, leading to desialylation of key membrane glycoproteins through NEU1-mediated mechanisms, thereby affecting monocyte adhesion and migration on endothelial cells. These results highlight a potential regulatory role of EDP and ERC in monocyte recruitment to inflamed vascular sites and suggest new biological functions in elastin remodeling-related disorders.
Background Vascular aging is associated with remodeling of elastin, one of the main extracellular matrix component of the arterial wall, and production of elastin-derived peptides (EDP). These extracellular matrix degradation products have been shown to trigger biological activities through the elastin receptor complex (ERC) and data from the last decade have brought significant insights on the critical role played by its NEU1 subunit in the biological effects mediated by EDP and the ERC in vascular and metabolic diseases. Results Using a proteomic approach, we previously identified new potential interaction partners of membrane NEU1. Here, we validated the interaction between NEU1 and the beta(2) integrin in human monocytes and show that binding of EDP to the ERC leads to desialylation of beta(2) integrin through NEU1. A similar action mechanism was identified in human umbilical vein endothelial cells (HUVEC) for intercellular cell adhesion molecule-1 (ICAM-1). Importantly, these effects were associated with a significant increase in monocyte adhesion to endothelial cells and monocyte transendothelial migration. Conclusions These results demonstrate that membrane NEU1 sialidase interacts and modulates the sialylation levels of the beta(2) integrin and ICAM-1 through the ERC in monocytes and endothelial cells, respectively, and suggest that EDP and the ERC, through this newly identified common mode of action governed by NEU1, may be important regulators of circulating monocyte recruitment to inflamed vascular sites. Moreover, by its ability to interact with and to modulate the sialylation of key membrane glycoproteins through NEU1, new biological functions are anticipated for EDP and the ERC in elastin remodeling-associated disorders.
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