期刊
ADVANCED HEALTHCARE MATERIALS
卷 11, 期 12, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202102383
关键词
human platelet lysates; personalized medicine; sponges; tissue engineering; xeno-free cell culture
资金
- Fundacao da Ciencia e Tecnologia through the project BEAT [PTDC/BTMMAT/30869/2017]
- Fundacao da Ciencia e Tecnologia through the CICECO - Aveiro Institute of Materials [UIDB/50011/2020, UIDP/50011/2020, LA/P/0006/2020]
- Fundacao da Ciencia e Tecnologia [SFRH/BD/144520/2019, 2020.01647.CEECIND]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/144520/2019] Funding Source: FCT
This study introduces a novel human protein-derived porous scaffold produced from platelet lysates, which serves as a platform for xeno-free 3D cell culture. The results show that the scaffold has tunable morphology and mechanical properties, making it suitable for human cell culture without the need for animal-derived supplements.
Extracellular matrix and protein-based biomaterials emerge as attractive sources to produce scaffolds due to their great properties regarding biocompatibility and bioactivity. In addition, there are concerns regarding the use of animal-derived supplements in cell culture not only due to the risk of transmission of xenogeneic contaminants and antigens but also due to ethical issues associated with collection methods. Herein, a novel human protein-derived porous scaffold produced from platelet lysates (PL) as platform for xeno-free 3D cell culture has been proposed. Human PL are chemically modified with methacryloyl groups (PLMA) to make them photocrosslinkable and used as precursor material to produce PLMA-based sponges. The herein reported human-based sponges have highly tunable morphology and mechanical properties, with an internal porous structure and Young's modulus dependent on the concentration of the polymer. Human adipose-derived stem cells (hASCs) are cultured on top of PLMA sponges to validate their use for 3D cell culture in xeno-free conditions. After 14 days hASCs remained viable, and results show that cells are able to proliferate during time even in the absence of animal-derived supplementation. This study reveals for the first time that such scaffolds can be promising platforms for culture of human cells avoiding the use of any animal-derived supplement.
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