期刊
ADVANCED HEALTHCARE MATERIALS
卷 11, 期 10, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202101605
关键词
fluorescence pH sensing; hydrogels; imaging; oxygen sensing; phosphorescence quenching; photography; polyamine porphyrin
资金
- SPIE
- Franz Hillenkamp Fellowship
Sensor-integrated wound dressings provide quantitative insights into tissue characteristics and enable easy monitoring of wound healing. This study presents a cost-effective method to quantify oxygen and pH in a hydrogel dressing using a single photograph. The luminescence of a dendritic polyamine Pt-porphyrin and fluorescein conjugate responds to oxygen and pH, respectively, and can be captured using a modified DSLR camera for image analysis.
Sensor-integrated wound dressings are emerging tools applicable to a wide variety of medical applications from emergency triage to at-home monitoring. Uncomfortable, unnecessary wound dressing changes may be avoided by providing quantitative insight into tissue characteristics related to wound healing such as tissue oxygenation, pH, and exudate/transudate volume. Here, a simple cost-effective methodology for quantifying oxygen and pH in a swellable hydrogel dressing using a single photograph is presented. The red and green luminescence of a novel dendritic polyamine Pt-porphyrin and fluorescein conjugate quantitatively responds to oxygen and pH, respectively, and enables robust sensing. The porphyrin conjugate, when combined with a four-arm star polyethylene glycol (PEG) amine polymer, rapidly crosslinks at room temperature with an N-hydroxysuccinimide (NHS)-PEG crosslinker to form a color-changing hydrogel dressing with tunable swelling capabilities applicable to a variety of wound environments. An inexpensive digital single-lens reflex (DSLR) camera modified with bandpass filters captures the hydrogel luminescence using simple macroscopic photography, and conversion to HSB colorspace allows for intensity-independent image analysis of the hydrogels' dual modality response. The hydrogel formulation exhibits a robust and validated visible red-orange-green traffic light spectrum in response to oxygen changes, regardless of swelling state, pH, or autofluorescence from skin, thereby enabling the clinician friendly naked-eye feedback.
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