期刊
ADVANCED HEALTHCARE MATERIALS
卷 11, 期 1, 页码 -出版社
WILEY
DOI: 10.1002/adhm.202100684
关键词
cancer metastasis; experimental methods; extracellular matrix; specialized bioreactors
资金
- European Research Council [ERC-2015-CoG-682881-MATRICAN]
- Lundbeck Foundation [R286-2018-621]
- Hallas MOller Stipend from the Novo Nordisk Foundation
- Innovation Fund Denmark Grand Solutions project [6153-00015B]
- Danish Cancer Society [R204-A12445, R204-A12454]
- Ragnar Soderberg Foundation [N91/15]
- Swedish cancer society, Cancerfonden [CAN 2016/783, 19 0632]
- Swedish Research Council [2017-03389]
- German Cancer Aid
- Swedish Research Council [2017-03389] Funding Source: Swedish Research Council
Metastatic cancer spread is responsible for most cancer-related deaths. Through the use of a bioreactor and organ ECM scaffolds, it is possible to mimic cell behavior within complex native ECM, aiding in the study of cell signaling and disease modeling.
Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell-cell and cell-ECM interplay and to model diseases in a controllable organ-specific system ex vivo.
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