期刊
ACS SYNTHETIC BIOLOGY
卷 10, 期 11, 页码 2784-2795出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.1c00315
关键词
plant natural products; synthetic biology; gene mining; microorganisms; candidate gene identification
资金
- National Key R&D Program of China [2021YFC2100500, 2019YFA0904104]
- Fundamental Research Funds for the Central Universities
Plant natural products (PNPs) have wide applications in various fields and researchers have been exploring the unknown biosynthetic pathways of PNPs for large-scale production, utilizing omics data and advanced technologies to identify candidate genes and characterize putative genes in heterologous hosts.
Plant natural products (PNPs) represent a vast and diverse group of natural products, which have wide applications such as emulsifiers in cosmetics, sweeteners in foods, and active ingredients in medicines. Large-scale production of certain PNPs (e.g., artemisinin, taxol) has been implemented by reconstruction of biosynthetic pathways in heterologous hosts. However, unknown biosynthetic pathways greatly restrict wide applications of heterologous production of PNPs of interest. With the rapid development of sequencing and multiomics analysis technologies, huge amounts of omics data, i.e., genomics, transcriptomics, and proteomics, have been deposited in public databases, which is a precious resource for identification of the unknown biosynthetic pathway of PNPs. Herein, we have enumerated the approaches which have been widely used to screen candidate genes involved in the biosynthesis of PNPs of interest. We also discuss recent developments in the characterization of putative genes and elucidation of the complete biosynthetic pathway in heterologous hosts.
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