4.7 Article

Programmable Fusion and Differentiation of Synthetic Minimal Cells

期刊

ACS SYNTHETIC BIOLOGY
卷 11, 期 2, 页码 855-866

出版社

AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.1c00519

关键词

cell-free; synthetic cell; genetic circuit; artificial life; TxTl; liposome

资金

  1. Hackett Royalty Fund award
  2. NIH [5 R 0 1 M H 1 1 4 0 3 1-0 2]
  3. NSF [1840301]
  4. NASA [CON00000065217]
  5. Semiconductor Research Corporation [2018-SB-2837-C]
  6. John Templeton Foundation Exploring the Informational Transitions Bridging Inorganic Chemistry and Minimal Life awards
  7. Div Of Molecular and Cellular Bioscience
  8. Direct For Biological Sciences [1840301] Funding Source: National Science Foundation

向作者/读者索取更多资源

Synthetic cells can mimic the complexities of live cells with less noise. This study demonstrates key functionalities of synthetic cells and provides tools for engineering and research.
Synthetic cells can mimic the intricate complexities of live cells, while mitigating the level of noise that is present natural systems; however, many crucial processes still need to be demonstrated in synthetic cells to use them to comprehensively study and engineer biology. Here we demonstrate key functionalities of synthetic cells previously available only to natural life: differentiation and mating. This work presents a toolset for engineering combinatorial genetic circuits in synthetic cells. We demonstrate how progenitor populations can differentiate into new lineages in response to small molecule stimuli or as a result of fusion, and we provide practical demonstration of utility for metabolic engineering. This work provides a tool for bioengineering and for natural pathway studies, as well as paving the way toward the construction of live artificial cells.

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