期刊
ACS SYNTHETIC BIOLOGY
卷 11, 期 1, 页码 353-365出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.1c00473
关键词
Streptomyces; sigma(hrdB); PkasO* mutant; metabolic regulation
资金
- National Natural Science Foundation of China [21776075]
- Chinese Postdoctoral Science Foundation [2020M671021]
- Natural Science Foundation of Shanghai [20ZR1415100]
The StSS system, consisting of two functional modules, effectively improves the production efficiency of metabolites in Streptomyces. By allowing continuous production processes to span different metabolic stages, it successfully enhances the yield of certain metabolites.
Streptomyces species possess strong secondary metabolism, the switches of which from the primary metabolism are complex and thus a challenge to holistically optimize their productivities. To avoid the complex switches and to reduce the limitations of different metabolic stages on the synthesis of metabolites, we designed a Streptomyces self-sustained system (StSS) that contains two functional modules, the primary metabolism module (PM) and the secondary metabolism module (SM). The PM includes endogenous housekeeping sigma factor sigma(hrdB) and sigma(hrdB)-dependent promoters, which are used to express target genes in the primary metabolism phase. SM consists of the expression cassette of sigma(hrdB) under the control of a secondary metabolism promoter, which maintains continuous activity of the sigma(hrdB)-dependent promoters in the secondary metabolism phase. As a proof-of-principle, the StSS was used to boost the production of some non-toxic metabolites, including indigoidine, undecylprodigiosin (UDP), ergothioneine, and avermectin, in Streptomyces. All these metabolites can undergo a continuous production process spanning the primary and secondary metabolism stages instead of being limited to a specific stage. Scale-up of UDP fermentation in a 4 L fermentor indicated that the StSS is a stable and robust system, the titer of which was enhanced to 1.1 g/L, the highest at present. This study demonstrated that the StSS is a simple but powerful strategy to rationally engineer Streptomyces cell factories for the efficient production of non-toxic metabolites via reconstructing the relationships between primary and secondary metabolism.
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