期刊
ACS SYNTHETIC BIOLOGY
卷 10, 期 12, 页码 3435-3444出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.1c00378
关键词
Saccharomyces cerevisiae; copper-inducible system; Gal80; degradation tag; dynamic control
资金
- National Natural Science Foundation of China [32001032]
- Natural Science Foundation of Jiangsu Province, China [BK20200946]
- China Postdoctoral Science Foundation [2020M671614]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [20KJD416003]
- Innovative and Entrepreneurial Talent of Jiangsu Province
- Lvyang Jinfeng Talent Attracting Plan of Yangzhou
A novel copper-inducible gene expression system, CuIGR, has been developed in S. cerevisiae for improved biosynthetic efficiency. This system, utilizing Gal4 and Gal80 regulators, showed stronger regulation compared to native promoters, leading to increased expression of EGFP and lycopene production with copper induction. The CuIGR system provides a powerful tool for gene expression control in yeast cell factories for enhanced biosynthesis of valuable products.
Dynamic regulation of gene expression to decouple growth and production has been proven to be effective for improving the biosynthetic efficiency of microbial cell factories. However, the number of efficient regulatory systems available for regulation of Saccharomyces cerevisiae is limited. In the present study, a novel copper-inducible gene expression system (CuIGR) composed of the copper-induced transcriptional activator Gal4 and the copper-inhibited repressor Gal80 was constructed in S. cerevisiae. When Gal80 was fused with a N-degron tag (K15), the resulting CuIGR4 system exhibited the most stringent regulation of gene expression driven by GAL1/2/7/10 promoters. As compared to the native Cu2+-inducible CUP1 promoter, the CuIGR4 system amplified the response to copper by as much as 2.7 folds, resulting in 72-fold induction of EGFP expression and a 33-fold change in lycopene production (3-100 mg/L) with addition of 20 mu M copper. This newly developed copper-inducible system provides a powerful tool for gene expression control in S. cerevisiae, which is expected to be widely applicable in the regulation of yeast cell factories for enhanced biosynthesis of valuable products.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据