4.7 Article

Epitachophoresis is a novel versatile total nucleic acid extraction method

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SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -

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NATURE PORTFOLIO
DOI: 10.1038/s41598-021-02214-1

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  1. Roche Sequencing Solutions (Pleasanton, U.S.A.)
  2. Institute of Analytical Chemistry, Czech Academy of Sciences in Brno, Czech Republic [RVO 68081715]

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Epitachophoresis is a versatile extraction system capable of isolating DNA and RNA from a wide range of nucleic acids, and the quality of extracted miRNA, mRNA, and gDNA was confirmed by downstream Next-Generation Sequencing.
Epitachophoresis is a novel next generation extraction system capable of isolating DNA and RNA simultaneously from clinically relevant samples. Here we build on the versatility of Epitachophoresis by extracting diverse nucleic acids ranging in lengths (20 nt-290 Kbp). The quality of extracted miRNA, mRNA and gDNA was assessed by downstream Next-Generation Sequencing.

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