期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-99046-w
关键词
-
资金
- Estonian Research Council [PUT 1371]
- EMBO Installation grant [3573]
- European Regional Development Fund [15-0012 GENTRANSMED]
- Estonian Center of Genomics/Roadmap II project [16-0125]
- European Regional Development Fund (Smart specialization PhD scholarship)
- European Union through Horizon 2020 research and innovation programme [81064]
- European Union through European Regional Development Fund [MOBEC008]
Colorectal cancer is a challenging public health problem and successful treatment depends on the stage at diagnosis. This study demonstrates that analyzing microbiome in FIT tubes for CRC screening programs is feasible and may improve the sensitivity of CRC detection.
Colorectal cancer (CRC) is a challenging public health problem which successful treatment depends on the stage at diagnosis. Recently, CRC-specific microbiome signatures have been proposed as a marker for CRC detection. Since many countries have initiated CRC screening programs, it would be useful to analyze the microbiome in the samples collected in fecal immunochemical test (FIT) tubes for fecal occult blood testing. Therefore, we investigated the impact of FIT tubes and stabilization buffer on the microbial community structure evaluated in stool samples from 30 volunteers and compared the detected communities to those of fresh-frozen samples, highlighting previously published cancer-specific communities. Altogether, 214 samples were analyzed by 16S rRNA gene sequencing, including positive and negative controls. Our results indicated that the variation between individuals was greater than the differences introduced by the collection strategy. The vast majority of the genera were stable for up to 7 days. None of the changes observed between fresh-frozen samples and FIT tube specimens were related to previously identified CRC-specific bacteria. Overall, we show that FIT tubes can be used for profiling the microbiota in CRC screening programs. This circumvents the need to collect additional samples and can possibly improve the sensitivity of CRC detection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据