期刊
SCIENTIFIC REPORTS
卷 11, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-021-00229-2
关键词
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资金
- Department of Science and Innovation (DSI)
- National Research Foundation (NRF) of South Africa under the Professional Development Programme (PDP)
- International Atomic Energy Agency [IAEA CRP E35010 (22248)]
- iThemba LABS
- BELSPO/NRF project [BELS180425324336]
The study showed that HSPCs exhibit higher DNA damage induction after neutron irradiation, leading to increased apoptosis rate and levels of cytogenetic damage, which contribute to a higher risk of leukemogenesis.
The radiosensitivity of haematopoietic stem and progenitor cells (HSPCs) to neutron radiation remains largely underexplored, notwithstanding their potential role as target cells for radiation-induced leukemogenesis. New insights are required for radiation protection purposes, particularly for aviation, space missions, nuclear accidents and even particle therapy. In this study, HSPCs (CD34(+)CD38(+) cells) were isolated from umbilical cord blood and irradiated with Co-60 gamma-rays (photons) and high energy p(66)/Be(40) neutrons. At 2 h post-irradiation, a significantly higher number of 1.28 +/- 0.12 gamma-H2AX foci/cell was observed after 0.5 Gy neutrons compared to 0.84 +/- 0.14 foci/cell for photons, but this decreased to similar levels for both radiation qualities after 18 h. However, a significant difference in late apoptosis was observed with Annexin-V+/PI+ assay between photon and neutron irradiation at 18 h, 43.17 +/- 6.10% versus 55.55 +/- 4.87%, respectively. A significant increase in MN frequency was observed after both 0.5 and 1 Gy neutron irradiation compared to photons illustrating higher levels of neutron-induced cytogenetic damage, while there was no difference in the nuclear division index between both radiation qualities. The results point towards a higher induction of DNA damage after neutron irradiation in HSPCs followed by error-prone DNA repair, which contributes to genomic instability and a higher risk of leukemogenesis.
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