A highly selective and sensitive spectrofluorimetric method for the assessment of 3-nitrotyrosine in serum using (Eu(TTA)3Phen) photo probe

A simple, accurate and fast method was developed for the assessment of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE) using a (Eu(TTA)(3)Phen) photo probe. 3-Nitrotyrosine can remarkably quench the luminescence intensity of the (Eu(TTA)(3)Phen) complex in DMSO at pH = 9 and lambda(em) = 617 nm. The quenching of the luminescence intensity of (Eu(TTA)(3)Phen) complex particularly the electrical emission band at lambda(em) = 617 nm is used for the assessment of 3-nitrotyrosine in different serum samples of patients with liver cirrhosis.

Automated summary

A simple, accurate, and fast method using a (Eu(TTA)(3)Phen) photo probe was developed to assess the potential of 3-nitrotyrosine as a biomarker for the early diagnosis of liver cirrhosis with minimal hepatic encephalopathy (MHE).