期刊
NUTRIENTS
卷 13, 期 11, 页码 -出版社
MDPI
DOI: 10.3390/nu13114066
关键词
diabetes mellitus; Reynoutria; Polygoni cuspidati rhizoma; polyphenols; protein glycation; advanced glycation endproducts; fructosamine; protein oxidation; amyloid aggregation
资金
- Wroclaw Medical University subvention grant [D030.21.029]
- Scientific Board of Pharmaceutical Sciences at the Wroclaw Medical University
Compounds extracted from the rhizomes of Japanese, Giant, and Bohemian knotweeds show promising antiglycoxidative potential for the prevention and complementary treatment of diabetes mellitus, with some fractions exhibiting comparable or stronger activity than the reference drug aminoguanidine.
Hyperglycemia, when sustained over a long time in diabetes mellitus (DM), leads to biochemical and cellular abnormalities, primarily through the formation of advanced glycation end-products (AGEs). In the treatment of diabetes, beside blood-sugar-lowering medications, a consumption of herbal products that can inhibit the AGEs' formation is recommended. This study investigated the in vitro antiglycoxidative potential of extracts and fractions from the rhizomes of Japanese, Giant, and Bohemian knotweeds (Reynoutria japonica (Houtt.), R. sachalinensis (F. Schmidt) Nakai, and R.x bohemica Chrtek et Chrtkova). Their effects on glycooxidation of bovine and human serum albumin were evaluated by incubation of the proteins with a mixture of glucose and fructose (0.5 M) and 150 mu g/mL of extract for 28 days at 37 & DEG;C, followed by measuring early and late glycation products, albumin oxidation (carbonyl and free thiol groups), and amyloid-beta aggregation (thioflavin T and Congo red assays). The highest antiglycoxidative activity, comparable or stronger than the reference drug (aminoguanidine), was observed for ethyl acetate and diethyl ether fractions, enriched in polyphenols (stilbenes, phenylpropanoid disaccharide esters, and free and oligomeric flavan-3-ols). In conclusion, the antiglycoxidative compounds from these three species should be further studied for potential use in the prevention and complementary treatment of DM.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据