期刊
NUTRIENTS
卷 13, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/nu13124275
关键词
vitamin A; retinoic acid; vitamin A supplementation; neonatal rats; intestine; tracer kinetics
资金
- Pennsylvania State University Graduate Program in Nutritional Sciences, NIH [HD-066982]
- Dorothy Foehr Huck Endowment
The study investigated the unique effect of retinoic acid (RA) on intestinal vitamin A (VA) kinetics by administering an RA dose before VA supplementation. The results showed that RA pretreatment could stimulate the influx of supplemental VA into the intestine, leading to increased VA accumulation in neonatal rats' intestines.
Given that combined vitamin A (VA) and retinoic acid (RA) supplementation stimulated the intestinal uptake of plasma retinyl esters in neonatal rats, we administrated an RA dose as a pretreatment before VA supplementation to investigate the distinct effect of RA on intestinal VA kinetics. On postnatal days (P) 2 and 3, half of the pups received an oral dose of RA (RA group), while the remaining received canola oil as the control (CN). On P4, after receiving an oral dose of H-3-labeled VA, pups were euthanized at selected times (n = 4-6/treatment/time) and intestine was collected. In both CN and RA groups, intestinal VA mass increased dramatically after VA supplementation; however, RA-pretreated pups had relatively higher VA levels from 10 h and accumulated 30% more VA over the 30-h study. Labeled VA rapidly peaked in the intestine of CN pups and then declined from 13 h, while a continuous increase was observed in the RA group, with a second peak at 10 h and nearly twice the accumulation of H-3-labeled VA compared to CN. Our findings indicate that RA pretreatment may stimulate the influx of supplemental VA into the intestine, and the increased VA accumulation suggests a potential VA storage capacity in neonatal intestine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据