期刊
NUTRIENTS
卷 14, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/nu14030723
关键词
dendritic cells (DCs); probiotics; TLR-ligands; T cells
Lipoteichoic acid (LTA) from different Gram-positive bacterial sources has varying immune effects. Lacticaseibacillus rhamnosus GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mice. In this study, the immunomodulatory effects of LGG-LTA on dendritic cells (DC) and T cells were evaluated in vitro and in the gut-associated lymphoid tissue (GALT). The results showed that LGG-LTA activated DC and T cells, promoting Th1-biased immune response after oral LTA administration.
Lipoteichoic acid (LTA) from Gram-positive bacteria exerts different immune effects depending on the bacterial source from which it is isolated. Lacticaseibacillus rhamnosus GG LTA (LGG-LTA) oral administration reduces UVB-induced immunosuppression and skin tumor development in mice. In the present work, we evaluate the immunomodulatory effect exerted by LGG-LTA in dendritic cells (DC) and T cells, both in vitro and in the gut-associated lymphoid tissue (GALT). During cell culture, LTA-stimulated BMDC increased CD86 and MHC-II expression and secreted low levels of pro and anti-inflammatory cytokines. Moreover, LTA-treated BMDC increased T cell priming capacity, promoting the secretion of IL-17A. On the other hand, in orally LTA-treated mice, a decrease in mature DC (lamina propria and Peyer's patches) was observed. Concomitantly, an increase in IL-12p35 and IFN-gamma transcription was presented (lamina propria and Peyer's Patches). Finally, an increase in the number of CD103+ DC was observed in Peyer's patches. Together, our data demonstrate that LGG-LTA activates DC and T cells. Moreover, we show that a Th1-biased immune response is triggered in vivo after oral LTA administration. These effects justify the oral LTA activity previously observed.
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