期刊
NUTRIENTS
卷 13, 期 12, 页码 -出版社
MDPI
DOI: 10.3390/nu13124290
关键词
Gongjin-dan; hydrogen peroxide; cortical neuron; Sirtuin1; nerve regeneration; neuroprotection; synapse; nerve growth factor; brain-derived neurotrophic factor
资金
- Traditional Korea Medicine Research and Development Program of the Korean Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare, Republic of Korea [HF21C0100]
GJD, a multiherbal formula, shows potential for preventing neurological diseases by ameliorating oxidative damage-induced neuronal death through Sirtuin1 activation, which also accelerates axon elongation and synapse formation, indicating a neuroprotective effect.
Gongjin-dan (GJD) is a multiherbal formula produced from 10 medicinal herbs and has been traditonally used as an oriental medicine to treat cardiovascular diseases, alcoholic hepatitis, mild dementia, and anemia. Additionally, increasing evidence suggests that GJD exerts neuroprotective effects by suppressing inflammation and oxidative stress-induced events to prevent neurological diseases. However, the mechanism by which GJD prevents oxidative stress-induced neuronal injury in a mature neuron remains unknown. Here, we examined the preventive effect and mechanism of GJD on primary cortical neurons exposed to hydrogen peroxide (H2O2). In the neuroprotection signaling pathway, Sirtuin1 is involved in neuroprotective action as a therapeutic target for neurological diseases. After pre-treatment with GJD at three concentrations (10, 25, and 50 mu g/mL) and stimulation by H2O2 (30 mu M) for 24 h, the influence of GJD on Sirtuin1 activation was assessed using immunocytochemistry, real-time PCR, western blotting, and flow cytometry. GJD effectively ameliorated H2O2-induced neuronal death against oxidative damage through Sirtuin1 activation. In addition, GJD-induced Sirtuin1 activation accelerated elongation of new axons and formation of synapses via increased expression of nerve growth factor and brain-derived neurotrophic factor, as well as regeneration-related genes. Thus, GJD shows potential for preventing neurological diseases via Sirtuin1 activation.
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