期刊
NUTRIENTS
卷 14, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/nu14040745
关键词
hesperetin; advanced glycation end-products; Alzheimer's disease; SH-SY5Y cells; endoplasmic reticulum stress
资金
- Ministry of Science and Technology of Taiwan [MOST 110-2320-B-127-001]
The study found that hesperetin, a citrus flavonoid, can counteract the pathophysiological changes of Alzheimer's disease induced by advanced glycation end-products (AGEs). The underlying mechanisms include reducing oxidative stress and apoptosis, regulating protein expression, and decreasing endoplasmic reticulum stress.
The present study investigates whether hesperetin, a citrus flavonoid, can encounter advanced glycation end-product (AGE)-induced Alzheimer's disease-like pathophysiological changes with the underlying mechanisms. SH-SY5Y cells pretreated with hesperetin before stimulation with AGEs (200 mu g/mL) were assessed in the following experiments. Hesperetin (40 mu mol/L) elevated the reduced cell viability induced by AGEs. Hesperetin ameliorated reactive oxygen species overproduction and the downregulation of superoxide dismutase, glutathione peroxidase, and catalase, triggered by AGEs. Amyloid precursor protein upregulation, accompanied by the increased production of A beta, caused by AGEs, was reversed by hesperetin. However, hesperetin lowered beta-site APP-cleaving enzyme 1 expression, inducing insulin-degrading and neprilysin expression. In addition, hesperetin downregulated the expressions of the AGEs-induced endoplasmic reticulum (ER) stress proteins, including 78-kDa glucose-regulated protein and C/EBP homologous protein, and lowered the phosphorylation of protein kinase R-like ER kinase and activating transcription factor 4. Hesperetin-pretreated cells had a minor apoptotic DNA fragmentation. Hesperetin is able to upregulate Bcl-2 protein expression, downregulate Bax expression, and decrease caspase-12/-9/-3 activity as well, indicating that it inhibits ER stress-mediated neuronal apoptosis. There is a similar effect between hesperetin and positive rosiglitazone control against A beta aggravation of SH-SY5Y cell injury induced by AGEs. Thus, hesperetin might be a potential agent for treating glycation-induced A beta neurotoxicity.
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