期刊
NUTRIENTS
卷 14, 期 2, 页码 -出版社
MDPI
DOI: 10.3390/nu14020328
关键词
flavonoids; gut microbiota; metabolite; 3-hydroxyphenylacetic acid; blood pressure; vasorelaxation; coronary; artery; rat; pig
资金
- Czech Research Health Council [NU21-02-00135]
- Charles University [GA UK 136120-C3, SVV 260 549]
- [136120-C3]
Regular intake of polyphenol-rich food can prevent cardiovascular diseases. A metabolite of polyphenols called 3-hydroxyphenylacetic acid (3-HPAA) decreases blood pressure by relaxing blood vessels, which might be mediated by the release of nitric oxide from endothelial cells.
Regular intake of polyphenol-rich food has been associated with a wide variety of beneficial health effects, including the prevention of cardiovascular diseases. However, the parent flavonoids have mostly low bioavailability and, hence, their metabolites have been hypothesized to be bioactive. One of these metabolites, 3-hydroxyphenylacetic acid (3-HPAA), formed by the gut microbiota, was previously reported to exert vasorelaxant effects ex vivo. The aim of this study was to shed more light on this effect in vivo, and to elucidate the mechanism of action. 3-HPAA gave rise to a dose-dependent decrease in arterial blood pressure when administered i.v. both as a bolus and infusion to spontaneously hypertensive rats. In contrast, no significant changes in heart rate were observed. In ex vivo experiments, where porcine hearts from a slaughterhouse were used to decrease the need for laboratory animals, 3-HPAA relaxed precontracted porcine coronary artery segments via a mechanism partially dependent on endothelium integrity. This relaxation was significantly impaired after endothelial nitric oxide synthase inhibition. In contrast, the blockade of SKCa or IKCa channels, or muscarinic receptors, did not affect 3-HPAA relaxation. Similarly, no effects of 3-HPAA on cyclooxygenase nor L-type calcium channels were observed. Thus, 3-HPAA decreases blood pressure in vivo via vessel relaxation, and this mechanism might be based on the release of nitric oxide by the endothelial layer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据