4.7 Article

Satellite Cells Exhibit Decreased Numbers and Impaired Functions on Single Myofibers Isolated from Vitamin B6-Deficient Mice

期刊

NUTRIENTS
卷 13, 期 12, 页码 -

出版社

MDPI
DOI: 10.3390/nu13124531

关键词

sarcopenia; vitamin B6; skeletal muscle; single myofibers; satellite cells; proliferation; self-renewal; amino acids; carnosine

资金

  1. Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT, Tokyo) [21K14804]
  2. Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA)
  3. Grants-in-Aid for Scientific Research [21K14804] Funding Source: KAKEN

向作者/读者索取更多资源

Research indicates that vitamin B6 deficiency reduces the number of dormant satellite cells in mouse muscles, inhibiting satellite cell proliferation and self-renewal during myogenesis.
Emerging research in human studies suggests an association among vitamin B6, sarcopenia, and muscle strength. However, very little is known regarding its potential role at the cellular level, especially in muscle satellite cells. Therefore, to determine whether vitamin B6 affects the satellite cells, we isolated single myofibers from muscles of vitamin B6-deficient and vitamin B6-supplemented mice. Subsequently, we subjected them to single myofiber culture and observed the number and function of the satellite cells, which remained in their niche on the myofibers. Prior to culture, the vitamin B6-deficient myofibers exhibited a significantly lower number of quiescent satellite cells, as compared to that in the vitamin B6-supplemented myofibers, thereby suggesting that vitamin B6 deficiency induces a decline in the quiescent satellite cell pool in mouse muscles. After 48 and 72 h of culture, the number of proliferating satellite cells per cluster was similar between the vitamin B6-deficient and -supplemented myofibers, but their numbers decreased significantly after culturing the myofibers in vitamin B6-free medium. After 72 h of culture, the number of self-renewing satellite cells per cluster was significantly lower in the vitamin B6-deficient myofibers, and the vitamin B6-free medium further decreased this number. In conclusion, vitamin B6 deficiency appears to reduce the number of quiescent satellite cells and suppress the proliferation and self-renewal of satellite cells during myogenesis.

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